INTEMA   05428
INSTITUTO DE INVESTIGACIONES EN CIENCIA Y TECNOLOGIA DE MATERIALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vitro Endothelial Cell Response to Ionic Dissolution Products Released From a Lithium-doped 45S5 Bioactive Glass
Autor/es:
L. A. HARO DURAND, G. E. VARGAS, R. VERA MESONES, M. A. FANOVICH, M. P. ZAGO, A. BALDI, A. GORUSTOVICH
Lugar:
Boston
Reunión:
Congreso; 4th TERMIS World Congress; 2015
Institución organizadora:
4th TERMIS World Congress
Resumen:
As it has been established that lithium (Li) may perform functions inangiogenesis, the controlled and localized release of Li ions frombioactive glasses (BGs) is expected to provide a promising therapeuticalternative for regenerative medicine of vascularized tissues. The aimof this study was to assess the in vitro angiogenic effects of the ionicdissolution products (IDPs) from BGs in the SiO2-CaO-Na2O-P2O5(45S5) system and of those from 45S5 BG doped with 5 wt% Li2O(45S5.5Li). IDPs were obtained by incubating 45S5 BG or 45S5.5LiBG particles ( < 5 mm) inM199 growing medium for 72h on an orbitalshaker at 37C. The soluble ions (Li, Si, P, Ca, and Na) were determinedby ICP-MS. The results show, for the first time, that the IDPsfrom 45S5.5Li BG stimulated human umbilical vein endothelial cells(HUVECs) proliferation and migration via an activation of b-cateninsignaling. It was also shown that IDPs from 45S5.5Li BG could enhancein vitro HUVECs tubule formation and secretion of IGF1 andTGFb. The effects observed are attributed to the presence of 0.2mMLiin the IDPs. These findings are relevant to tissue engineering and regenerativemedicine because the IDPs from 45S5.5Li BG may act asinexpensive inorganic angiogenic agents providing a convenient alternativeto the application of conventional angiogenic growth factors.