Redox profiling of G. sulfurreducens biofilms by confocal Raman microscopy

L. ROBUSCHI; J. P. TOMBA; G. SCHROTT; S. BONANI; P. M. DESIMONE; J. P. BUSALMEN

Madrid

Congreso; 2nd European meeting of the international Society of Microbial Electrochemistry and Technology; 2014

Universidad de Alcala

Aiming at gaining information on structural and physiological features of electricityproducing biofilms, we have constructed an electrochemical cell that can be mounted on the stage of a microscope. The cell is designed to use thin film transparent electrodes, thus allowing the observation of biofilms in situ and in vivo through the electrode. Using this cell in conjunction with a confocal Raman microscope (CRM), the redox state of molecules at different focal planes of Geobacter sulfurreducens biofilms was explored. Upon polarization interruption there is a transient gradient inversion at the biofilm/electrode interface evidencing relocalization of the electrons across the biofilm matrix.