INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Polyunsaturated n-3 fatty acids (PUFAs) prevent the cardiac hypertrophy in hypertensive rats
Autor/es:
MAITE R. ZAVALA; OMAR VÉLEZ RUEDA; LUCRECIA LONGARZO; SABINA M. MATÉ; MARÍA J. ALMADA; MARÍA C. VILLA-ABRILLE
Lugar:
Mar del Plata
Reunión:
Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020
Resumen:
It has been demonstrated that supplementation with the two main polyunsaturated n-3 fatty acids (PUFAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) ,leads to modifications on the cardiac physiology. PUFAs can affect membrane´s lipid composition, as well as proteins? location and/or function. The Na+/H+ exchanger (NHE1) is an integral membrane protein involved in maintenance of intracellular pH (pHi). Its activity is regulated by allosteric site sensitivity for H+, phosphorylation and by union of ATP, lipids, growth factors in its cytoplasmic tail. The purpose of this work was to evaluate the effect of early supplementing in diet with EPA and DHA over the modulation of NHE1 activity and cardiac function in normo rats (Wistar, W) and spontaneous hypertensive (SHR).After weaning, the animals received orally EPA and DHA for three months (200 mg/kg body mass/day). Then we measured systolic pressure (SP) and different echocardiography parameters, which was used to calculated left ventricular mass index (LVMI), ejection fraction (EF%) and fractional shortening (FS%). The rats were sacrificed and obtain ventricular cardiomyocytes for measured the NHE1 activity.The SP was significantly greater in SHR compared to W. While the treatment with PUFAs did not affect the SP in SHR, we observed a significant reduction in LVMI.The NHE1 activity was measured as velocity of pHi recovery (dpHi/dt) after intracellular acidification. As is previously described, the NHE1 activity was significantly higher in SHR compared with W. NHE1 activity was not modified by the treatment with PUFA in W. However, NHE1 activity was significant decreased by PUFA in SHR, reaching values comparable with W.These preliminary results allow us to suggest that diet supplementation with PUFAs since early age in SHR prevents the development of cardiac hypertrophy, perhaps by decreasing NHE1 activity, without altering hemodynamic overload.