INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of copper overload on the survival of human derived cells in cultured (Hep G2 and A549).
Autor/es:
ARNAL NATHALIE; TACCONI DE ALANIZ MARÍA J.; MARRA CARLOS ALBERTO
Lugar:
San Miguel de Tucumán. Argentina
Reunión:
Congreso; XLV Reunión anual de la sociedad argentina de Investigación en Bioquímica y Biología Molecular.; 2009
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
Resumen Copper (Cu) may cause important alterations in the antioxidant defence system on human cell lines. In this work we reported  differential responses by Cu overload (0-180 nM/24 h) in HepG2 and A549 evaluated through various parameters involved in apoptosis or necrosis. For both lines, the rate of cellular growing was depressed in a concentration-dependent way which was more evident in HepG2. However, it showed shorter estimated mitosis times than A549. The changes were associated to an increased miliand microcalpaine activity which was higher for HepG2. Caspase-3 activity was raised at different doses (40 and 80 ìM Cu, for A459 and HepG2 respectively). LDH was measured as a function of Cu overload and demonstrated that necrosis is linear in the case of HepG2, while for A549 the response seems to be sigmoid. Cu was accumulated in both cells, being more important in the case of A549. These cells also exhibited a slow elevation of ceruloplasmine. The increase of metallothionein was more evident in HepG2. Results suggest that Cu overload induces both apoptosis and necrosis; however, in HepG2 the necrotic mechanism should be more prevalent. Modifications in cell survival induced by Cu may be important for the understanding of damage produced by involuntary exposition to this cation, and also in the ethiology of illnesses associated to Cu overload such as neurodegenerative disorders.