INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Why are spiders so fast? A small biochemical history
Autor/es:
LAINO ALDANA; CARRASCO-MIRANDA, JESUS S.; GARCIA FERNANDO; GARCIA-OROZCO KARINA; LOPEZ-ZAVALA ALONSO; SOTELO-MUNDO, ROGERIO R.
Reunión:
Congreso; XXI International Cangress of Arachnology; 2019
Resumen:
Spiders are characterized by being hunter animals, this implies that they must develop great speed to be able to catch their prey, and even jump on them, this characteristic is very well developed in spiders that do not generate web, as is the case of Polybetes pythagoricus. At present no records are found of how spiders get energy to make such movements so fast because the aerobic routes seem to be insufficient, but the most consistent hypothesis is that they obtain it through a enzime (arginine kinase). In this work, we studied for the first time in spiders (using Polybetes pythagoricus) arginine kinase (PpAK).The PpAK cDNA was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein with a calculated mass of 43 kDa. The sequence displays extensive similarity to other invertebrate arginine kinases (spider, horseshoe crab, crustaceans and acari). The apparent Michaelis-Menten kinetic constant (Km) and maximum velocity (Vmax) were 1.703 mM and 27.77 μmol.min-1.mg protein-1, respectively. The crystal structure showed with both ligand free and arginine binary complex were found in the open conformation with loop comprised from residues (310-320) fully disordered and not covering de active site as in the ternary complex reported for other guanidine kinases. Finally, these results explican contribute to knowledge of mechanistic details of arginine kinases function y reporta informacion relevante para las arañas en sitios de almacenamiento de datos cientificos como (GenBank MF001441) and protein data bank. (http://www.rcsb.org/pdb/explore/explore.do?structureId=5U92)