INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Mesenchymal stem cells therapy reversed hippocampal atrophy, neurodegeneration, loss of presynaptic proteins, reactive microglia and behavior impaired in a rat model of sporadic Alzheimer?s disease
Autor/es:
ZAPPA VILLAR, MF; LOPEZ HANOTTE, J; REGGIANI, PC; PARDO, J
Lugar:
Córdoba
Reunión:
Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
Sporadic Alzheimer?s disease (SAD) is a progressive neurodegenerative disorder with no efficient therapy. We are interested in developing therapeutic strategies to overcome the degenerative changes in SAD. In this context, we explored the neuroprotective effect of human mesenchymal stem cells (MSC), using a SAD rat model by intracerebroventricular injection of streptozotocin (icv-STZ). Animals were divided into 3 experimental groups: Sham, STZ and STZ+MSC. STZ and STZ+MSC received 3 mg/kg icv-STZ and, 24 days after, STZ+MSC received, every 18 days, 1x106 MSC in a tail vein. During the last two weeks until the end of the study (3 months post-icv-STZ), we performed different behavioral tests. Our results show that STZ treated rats were behaviorally impaired, whereas the STZ+MSC group improved its spatial memory and decreased the anxiety. Immunohistochemistry in the Stratum Radiatum (SR) of the hippocampus revealed that neurons, astrocytes and microglial cells were affected by STZ, and MSC therapy reversed the observed changes in neurons, microglial cells, and in the volume of the SR, previously atrophied by the STZ. Interestingly, Western Blots of hippocampal lysates on presynaptic proteins (SYT1, SYT2, SYP and SV2) and GABAergic neuron marker (GAD65/67) show decreased protein levels in the STZ group, whereas MSC therapy led to a recovery of SYT1, SYP and GAD65 levels. We conclude that MSC therapy is a suitable biological tool in neurodegenerative disorders.