INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FATTY ACIDS SIGNALING IN A DOPAMINERGIC NEURONAL MODEL
Autor/es:
ANDRES MARTIN TOSCANI; CHRISTOF LENZ; LISANDRO J. FALOMIR LOCKHART; MARÍA ALEJANDRA CARRERO RIVEROS; TIAGO OUTEIRO; EZEQUIEL GIMÉNEZ; HENNING URLAUB
Lugar:
Bariloche
Reunión:
Congreso; SISTAM 2018; 2018
Resumen:
Fatty acids (FAs) are classically associated with structural and metabolic roles. Recently, it has been shown that several lipids also exhibit regulatory functions, similarly as hormones or growth factors. These "lipokines" bind to specific receptors triggering second messenger´s systems and regulating gene expression. There are two families of FAs receptors: free fatty acid receptors (FFARs) and peroxisome proliferator-activated receptors (PPARs). Central nervous system is enriched in poly-unsaturated fatty acids (PUFAs) that participate in the regulation of membrane fluidity, axonal growth, development and inflammatory response. Alterations in lipid metabolism are associated with cognitive problems and neurodegenerative diseases, but the molecular mechanism behind them have not yet been described.The aim of the present study is to characterize the effect of FAs in the differentiation of SH-SY5Y cells, broadly used as model system for in vitro studies of dopaminergic neurons. SH-SY5Y cells can be differentiated to a more mature, neuron-like phenotype by incubation with retinoic acid (RA), with an increase in Akt expression and phosphorylation levels, but with a decrease in tyrosine hydroxilase (TH). We evaluated biochemical and morphological changes during differentiation, with and without supplementation with different FAs. We also studied FFAR1 and FFAR4 signaling employing natural and synthetic agonists through Ca release and Akt signaling pathway.Our results suggest a new role for FAs in neuronal differentiation. Characterization of the molecular events related to the activation of lipid receptors in the nervous system will provide us a framework to further understand their role in neurophysiology, and it could be explored for enhancing neuronal surviving under stress conditions in the treatment of neurodegenerative diseases like Parkinson´s disease.