OXIDATIVE AND GENOTOXIC EFFECTS OF MONOTERPENES IN HUMAN NON-SMALL CELL LUNG CANCER A549 CELLS

RODENAK KLADNIEW B; LAVARÍAS S; CRESPO R; CASTRO MA; GARCÍA DE BRAVO M

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Monoterpenes (Mts) are volatile organic compounds mostly foundin the essential oils (EOs) of citrus fruits and aromatic plants, synthesizedas secondary metabolites with an antimicrobial effect. Invitro physicochemical assays have characterized most of them asantioxidants. However, in eukaryotic cells Mts can modify their redoxenvironment according to their chemical structure, the concentrationand time of exposure, as well as the type of cell evaluated. EOs arewidely proposed as anticancer natural compounds. We aim to testbiomarkers of oxidative stress such as lipids, DNA, and antioxidantenzymes in human non-small cell lung cancer A549 cells incubatedin different culture conditions and treated with antiproliferative concentrationsof the acyclic unsaturated oxygenated Mt geraniol (G)and mandarin Citrus reticulata peel oil (MPO) composed in a 95 %of unsaturated cyclic Mts. Cells were treated with 200 μM of G or 50,100, and 130 μl/L of MPO. Lipid peroxidation (LP) was measuredby TBARS assay, genotoxic activity by comet assay, and superoxidedismutase (SOD), catalase (CAT) and glutathione-S-transferase(GST) activities by spectrophotometric analysis. We observed thatG induced LP (p