Expansions of the cytochrome P450 gene superfamily are related to insecticide resistance in Triatoma infestans

MORICONI, DÉBORA E.; JUAREZ M.PATRICIA; CALDERÓN FERNÁNDEZ, GUSTAVO M.; DULBECCO, ANDREA B.; PEDRINI, NICOLÁS

Kolymbari

Congreso; EMBO conference: Molecular and population biology of mosquitoes and other disease vectors: vector and disease control; 2017

European Molecular Biology Organization (EMBO)

The insect integument, formed by the cuticle and the epidermal tissue, plays a fundamental role in insecticide resistance as a physical barrier. However, there is no information to date about the role of the integument from triatomine vectors in insecticide detoxification. In the genome of the triatomine Rhodnius prolixus a high expansion of cytochrome P450 subfamilies (CYP3093A and CYP4EM) was reported (1, 2). In this study, we describe new T. infestans integument genes that are part of these subfamilies. Also, we modeled the docking of deltamethrin to the active site in all members of these subfamilies both in R. prolixus and T. infestans. A differential expression analysis of these genes was performed in the integument of pyrethroid-resistant T. infestans nymphs. Finally, we knocked-down the two most highly expressed genes in resistant nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their potential participation in deltamethrin resistance