INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Restorative effects of hMSC therapy on spatial memory in senile rats
Autor/es:
ZAPPA-VILLAR MF; MAZZOLINI G; GARCIA MG; GOYA RG; LOPEZ-LEON M; MOREL GR; REGGIANI PC
Lugar:
Buenos Aires
Reunión:
Congreso; 2nd FALAN Congress; 2016
Institución organizadora:
FENS-IBRO
Resumen:
Brain aging is associated with a progressive increase in the incidence of neurodegenerative diseases and deterioration of spatial learning and memory in aging rats and humans. Here, we investigated the therapeutic potential of human adult bone marrow-derived mesenchymal stem cells (BM-MSCs) to treat cognitive impairment in Senile rats (27 months). Female rats were divided into 3 groups (N=8 each): Young-intact (3 months), Senile-Intact and Senile- MSC (intracerebroventricular injected with Dil-labeled human BM-MSCs). Using the Barnes maze we assessed hippocampus-dependent learning and spatial memory before and after cell injection. Additionally, we performed time-course studies for MSCs integration and viability in the brain and assessed a set of hippocampal cell markers.Human BM-MSC therapy significantly increased goal hole and goal sector exploration activity in senile rats as compared with intact counterparts. Immature neuron number in the hippocampal dentate gyrus (DG) fell sharply in the senile animals as compared with young counterparts and was comparable in the hippocampal DG of both old groups. Time-course studies (24 days) revealed that MSCs integrated into ependymal cell layer and occasionally in the brain parenchyma.The results suggest that human BM-MSC therapy partially reverses the decline in cognitive performance that occurs in senile rats. We conclude that human BM-MSC are a promising biological tool for the treatment of age-related spatial memory deficits.enviar mensaje