INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LFABP knockdown impairs fatty acid asymilation and metabolism in Caco-2 cells
Autor/es:
FALOMIR LOCKHART LJ; RODRIGUEZ SAWICKI L; FRANCHINI G; STORCH J; CÓRSICO B
Lugar:
Tucumán, Argentina
Reunión:
Congreso; XLV Reunión Annual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2009
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> The intestine assimilates and processes a large quantity of lipids incorporated with the diet. Lipids are assimilated mainly as free fatty acids (FA), cholesterol and monoacylglicerol to be then reesterified inside the enterocytes and secreted as Quilomicrons into the lymph. The classical functions attributed to FABP are the cytosolic buffer and transporter of hydrophobic ligands. Nevertheless, new functions have been suggested related to control of gene expression. It is suggested that intestinal FABP would facilitate the intracellular transport of FA and that they could also have a role in the regulation of lipid metabolism and other cellular processes. In this work we obtained a L-FABP knock-down model in Caco-2 cells by anti-mRNA overexpression. We analyzed the effect on the assimilation, metabolism and secretion of FA as well as on the cellular proliferation and differentiation processes. No compensation by I-FABP was observed in differentiated cells. Knock-down clones showed a marked decrease in oleate assimilation, while palmitate assimilation was increased. Differences in OA distribution were observed at short times, but they disappeared after hours and no change in oleate distribution was observed in secreted lipids. On the other hand, cell proliferation and differentiation were slowed. These results indicate that L-FABP is an important factor of enterocyte functionality.