- In vitro and in vivo evaluation of mandarin peel oil on lipid metabolism and tumor growth

PETERSON G; CRESPO R; POLO M; POLO M; CASTRO MA; GARCÍA DE BRAVO M ; CASTRO MA; GARCÍA DE BRAVO M ; PETERSON G; CRESPO R

Córdoba

Congreso; LII Reunión Anual de SAIB; 2016

SAIB

The aim of this work was to study the effects of mandarin (Citrus reticulata) peel oil (MPO) on lipid metabolism and cell proliferation. HepG2 and A549 human tumor culture cells were used for in vitro assays, and A549 cells were implanted in nude mice for in vivo assays. Mice were fed with a control or experimental (2.1 and 6.3 μl MPO/mouse/day) diet for 21 days. Systemic toxicity evaluation, tumor growth measurement, and histological analyses were performed. Radioactive lipid precursors, thin-layer chromatography, flow cytometry, and MTT and TUNEL assays were the methodology employed for in vitro and/or in vivo assays. Treated mice did not show significant differences in quantity of food intake, body and organ weight, serum activity of aspartate and alanine aminotransferases, and albumin content compared with controls. In tumor cells MPO: 1, decreased 14C-acetate incorporation into cholesterol, phospholipids, and triglycerides; 2, inhibited cell proliferation and cell cycle progression with an arrest at the G0 /G1 interphase (IC50= 116 μl MPO/L); 3, significantly diminished tumor growth (49%) with 6.3 μl MPO/mouse/day; and 4, increased apoptosis (0.5-1.7% in in vivo assaysand 9-30% in in vitro assays). Based on these results, we conclude that MPO has a great potential as a natural non-toxic hypolipogenic and chemotherapeutic agent.