Lipid metabolism in cancer cells: role of FABP

CÓRSICO B; VES LOSADA, A; GARCÍA KA; SCAGLIA N

Córdoba

Congreso; LII Reunión Anual de SAIB; 2016

SAIB

Cancer cells show a metabolic reprograming towards macromolecular synthesis, required for cellular growth. Fatty acid-binding proteins (FABPs) are intracellular proteins that bind hydrophobic ligands with high affinity. It has been proposed that FABPs could transport theirs ligands to the different compartments within the cell. FABP5 is overexpressed in several types of cancers, but its function in tumor metabolism is still unknown. We found that glucose and exogenous fatty acids alter FABP5 expression in A549 human lung adenocarcinoma cell. In addition, chemical inhibition of de novo fatty acid synthesis decreased FABP5 expression and cellular proliferation. Moreover, the siRNA-mediated reduction of FABP5 in A549 cells impaired fatty acid uptake and proliferation compared to non targeting control siRNA-transfected cells. The distribution of exogenous fatty acids among different lipid species was, however, unchanged. Thus, our results suggest that FABP5 expression is modulated by nutritional factors / metabolic conditions and it is essential for fatty acid uptake and proliferation in human lung cancer cells.