INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hydrocarbon assimilation in eurotialean and hypocrealean fungi: roles for CYP52 and CYP53 genes
Autor/es:
CARLA HUARTE BONNET; NICOLAS PEDRINI; JUAN CRUZ PONCE; MARIANELA SANTANA
Reunión:
Congreso; SAIB 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016
Resumen:
Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 has been regarded as the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. We performed a comparative analysis of CYP genes belonging to CYP52 and CYP53 families in Aspergillus niger, Penicillium chrysogenum, Beauveria bassiana and Metarhizium anisopliae. These species were able to assimilate both types of hydrocarbons, exhibiting a species-dependent modification in pH during this process. By modeling the molecular docking to the active site, we showed that both types of substrates are energetically favored for their binding to enzymes codified by fungal genes belonging to both CYP52 and CYP53 families. Gene expression analyses revealed that CYP53 members are highly induced by phenanthrene, but no induction was observed with n-alkanes. These findings suggest that the set of P450 enzymes involved is dependent on phylogeny, and reveal overlapping role but distinct substrate and expression specificities.