INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cytochrome P450 monooxigenase genes (CYP) associated with pyrethroid resistance in the Chagas disease vector Triatoma infestans
Autor/es:
PEDRINI, NICOLÁS; KUMAR, SURESH; SALAMANCA-MORENO, JHON A.; JUÁREZ, M. PATRICIA; CALDERÓN FERNÁNDEZ, GUSTAVO M.; FLEITA, WILMA
Lugar:
Orlando
Reunión:
Congreso; International Congress of Entomology; 2016
Institución organizadora:
Entomological Society of America
Resumen:
The blood-sucking bug Triatoma infestans (Hemiptera: Reduviidae) is the most widespread and relevant vector of Chagas disease in the southern region of South America. Residual spray with chemical insecticides has been a successful vector control strategy. However, after about 30 years of pyrethroid application, resistance foci are increasingly being documented in Argentina and Bolivia. Among genes involved in detoxification processes in insects, cytochrome P450 monooxigenases (CYP) are often associated with relevant roles in metabolic resistance. Using the Rhodnius prolixus genome information available, we characterized 33 CYP genes in the integument of T. infestans. Two of them, classified as members of CYP4EM and CYP3093A subfamilies, were highly expressed (18- and 67-fold induction, respectively) in deltamethrin-resistant fifth-instar nymphs compared with deltamethrin-susceptible specimens, as measured by qPCR. After modeling the molecular docking of deltamethrin to the predicted active sites, the pyrethroid binding was energetically favored for some members of CYP4 but not for CYP3093 genes. These results suggest that a combination of at least two mechanisms, i.e. induction of gene expression and modification of binding affinity to the active site, might be responsible for the high levels of deltamethrin resistance detected in T. infestans. Further studies including the silencing of candidate genes will help to understand the mechanisms involved in such resistance.