CADMIUN ALTERS THE LIPID METABOLISM IN RAT MAMMARY GLAND. EFFECT OF SOY PROTEIN

SANCHEZ; VASQUEZ; PLATEO; ARNAL, NATHALIE; ALVAREZ; GIMENEZ

Congreso; LI Reunión anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2015

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

We studied the effect of different diets on the lipid profile of rat mammary gland (MG). We also looked at the potential protective outcome of those diets under exposure to Cadmiun (Cd), which is a toxic element and an important environmental contaminant.4 female lots Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control) and the other, 15 ppm of Cd in the drinking water for 60 days. Lipids were extracted by Hexano:Isopropanol:BHT( 3v:2v:1%.) solvent. Total cholesterol (TC) and phospholipids (PL) were determined by colorimetric assay. Fatty acids (FA) were subjected to gas chromatography-mass spectrometry. HMG CoA Reductase (HMGCoAR) protein expression was determined by Western blot. Interestingly, our results show that Soy diet decreases TC and tend to increase PL in relation to Cas. Cd does not influence the level of TC and PL in either group. HMGCoAR expression is decreased by Cd in CAS fed rats as well as on both group of Soy diet. Regarding to the FA composition we observed that Cd decreases the amount of 18:2 y 18:3n3 in CAS group, which may suggest that Soy prevents the effect of Cd. Simultaneously, Soy decreases 16:0 compared to Cas. These findings indicate that Cd exposure alters the composition of lipids in MG and Soy protein diet may regulate these effects.