INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vivo and in vitro evaluation of lipid peroxidation by geraniol
Autor/es:
GALLE M; CASTRO MA; CRESPO R; GARCÍA DE BRAVO M
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual de SAIB; 2014
Institución organizadora:
SAIB
Resumen:
Abstract. We have previously demonstrated antitumor activity and antiatherogenic properties of geraniol (G). To evaluate the anti or pro-oxidant effect of G and its relationship with those processes we studied the most sensitive oxidative damage biomarker (lipid peroxidation) in tissues obtained from mice and also in human plasma LDL. To analyze the impact of dietary G in vivo we implanted A549 cells in nude mice and animals were fed a control or experimental (25; 50 or 75 mmol G/kg food) diet for 21 days after tumor implant. Liver, adipose tissue and tumors were removed from sacrificed mice. Additionally, plasma LDLs isolated from healthy human donors were preincubated with G (20-5000 μM) and oxidized with CuSO4. Lipid peroxidation in mice tissues and plasma LDL was measured by TBARS assay. Our results showed that G significantly decreased lipid peroxidation in adipose tissue (20-35 %) but enhanced it in tumor (300%). Nevertheless, G did not modify this parameter in mice livers. In vitro tests showed that low concentrations of G inhibited 30% of LDL lipid peroxidation whereas LDL treated with high doses showed no significant changes from control samples. These data demonstrated that G possesses anti or pro-oxidative effects depending on doses used as well as cell type. It suggests that G has a dual effect on redox balance with a great potential as cytotoxic and/or an antiatherogenic agent.