INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Evaluation of antiproliferative and antioxidant effect of isoprenoids for potential chemopreventive and/or chemotherapy use
Autor/es:
ROSANA CRESPO; MARÍA VICTORIA SOBERON; SABRINA LAVARÍAS; MARIANELA GALLE ; BORIS RODENAK KLADNIEW; SANDRA MONTERO VILLEGAS; AGUSTINA CASTRO; MÓNICA POLO; MARGARITA GARCÍA DE BRAVO
Reunión:
Congreso; 14th International Congress of Ethnopharmacology; 2014
Resumen:
Isoprenoids are naturally occurring organic chemicals, derived from five-carbon isoprene units, and they are present in the essential oils (EOs) of many aromatic plants. They have attracted interest for its potential antitumor and antioxidant properties. The objective of our work is to evaluate the effect of several monoterpenes as geraniol, limonene, cineole and linalool, and Citrus reticulate and Lippia alba EOs, in the cell cycle progression of tumor cells, as well as its antioxidant enzymatic activity. Several assays were performed on lung epithelial (A549) and hepatoma (HepG2) human cell lines. Cell viability and proliferation was evaluated by MTT assays and trypan blue exclusion method, cell cycle progression was analyzed by flow cytometry, effects on DNA integrity was assessed with the comet assay and lipid peroxidation by TBARS assay. Superoxide dismutase, catalase and glutathione - S -transferase activities were determined by spectrophotometric analyses. Our results showed that at low levels of these natural compounds, cell viability was increased while DNA damage, lipoperoxidation and the activity of the three enzymes analyzed were not induced. But at high doses, we found that they imposed oxidative stress, damaged DNA and lipid peroxidation, and inhibit cell cycle progression with an arrest occurring at the G0/G1 interphase. These data suggest that isoprenoids possess both pro- and antioxidative effects and they should be used as cytoprotective and/or cytotoxic agents.