INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
GPAT2, a gene involved in piRNA biogenesis, is epigenetically regulated and promotes tumoral phenotype of MDA-MB-231 cell line
Autor/es:
GARCIA FABIANI, MARIA BELEN; MONTANARO, MAURO ALDO; LACUNZA, EZEQUIEL; QUIROGA, IVANA YOSELI; ABBA, MARTIN; GONZALEZ BARO, MARIA DEL ROSARIO; PELLON MAISON, MAGALI
Lugar:
Smithfield, RI
Reunión:
Congreso; Gordon Research Conference ?Epigenetics Mechanisms and Implications?; 2013
Resumen:
GPAT2, a protein involved in piRNA biogenesis, is epigenetically regulated and promotes tumoral phenotype of MDA-MB-231 cell line The first step in de novo glycerolipid biosynthesis is catalyzed by glycerol-3-phosphate acyltransferase (GPAT). GPAT2, located in the outer mitochondrial membrane, is highly expressed in testis germ cells and in some cancer and cancer-derived cell lines thus being proposed as a member of the ?cancer-testis gene? family, which includes an heterogeneous group of proteins (CT antigens), expressed almost exclusively in normal testis and certain cancer cells . Also, It GPAT2 was recently found that GPAT2 is as essential for piRNA biogenesis. The aim of this study was to evaluate the phenotypic consequence of GPAT2 expression in cancer cells and to understand the mechanism by which GPAT2 is over expressed in human cancer.The aim of this study was to evaluate the mechanism and phenotypic consequences of GPAT2 upregulation in human cancer cells. By in silico analysis, we selected MDA-MB-231 as a tumorigenic cell model of tumorigenic cell line that express with a constitutively expression of GPAT2. A stable GPAT2 knock down (KD) MDA-MB-231 cell line, with only 5% of GPAT2 remaining expression, showed a 50% lower cell growth rate in MTT assays, a 90 % reduction of 90 % in the number of colonies in soft agar colony assay, and a 60% reduction in the percentage of closure in wound healing closing ability assay (60 % at 8 hours), compared to control scramble-shRNA expressing cells. Additionally, apoptotic cell to total cell ratio, was as determined by TUNEL assay, . At 30 min of staurosporin treatment, apoptosis was dramatically increased from 6.6 to 85.4 % in (control vs. GPAT2 KD MDA-MB-231 cells) after 30-min staurosporin treatment, and (p