INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Long-term expression of a synthetic gene encoding the anti-inflammatory peptide thymulin
Autor/es:
GR MOREL; BROWN, OA; SOSA, YE; HERENU, CB; GO ZUCCOLILLI; EL PORTIANSKY; DARDENNE, M; RODOLFO GUSTAVO GOYA
Lugar:
Huerta Grande, Cordoba
Reunión:
Congreso; VIII Taller Argentino de Neurociencias; 2006
Resumen:
Long-term nigral expression of a synthetic gene encoding the antiinflammatory peptide thymulin Morel Gustavo Ra, Brown Oscar Aa, Sosa Yolanda Ea, Hereñú Claudia Ba, Portiansky Enrique Lb, Zuccolilli Gustavo Oc, Goya Rodolfo Ga aInstitute for Biochemical Research-Histology B (INIBIOLP), Faculty of Medicine, Argentina; bInstitute of Pathology and cInstitute of Anatomy, School of Veterinary Sciences, UNLP, La Plata, Argentina     Abstract Thymulin is a thymic peptide with antiinflammatory activity in the brain. We constructed a recombinant adenoviral vector, RAd-FTS, expressing a synthetic DNA sequence encoding met-FTS, a biologically active analog of thymulin and used it for central gene transfer in rats. Thymulin concentration in  brain tissue was determined by bioassay. Reporter gene expression in the susbstantia nigra (SN) was quantitated by enzymohistochemistry or fluorescence microscopy using an appropriate image analysis software. Stereotaxic intranigral injection of RAd-FTS induced steady expression levels of met-FTS for at least 90  days, whereas expression of adenovirally-transferred reporter genes coding for green fluorescent protein fused to HSV thymidine kinase (GFP-TK)fus or E. coli β-galactosidase (β-gal), declined rapidly and was no longer detectable at post-injection day 14 and 30, respectively. We conclude that RAd-FTS constitutes a suitable biotechnological tool for the assessment of neuroprotective thymulin gene therapy in animal models of nigral dopaminergic neurodegeneration induced by pro-inflammatory agents.