INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Purification and characterization of lipovitellin from eggs of the wolf spider Schizocosa malitiosa
Autor/es:
LAINO ALDANA; CUNNINGHAM MONICA; COSTA FERNANDO; FERNANDO GARCIA
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; 2012
Resumen:
Vitellins are essential in ovigerous species for embryogenesis and viability of progeny, but in spiders, only have been described the egg lipovitellins in the species Polybetes pythagoricus, so there is virtually no information in arachnids. The aim of this work was to purify and characterize lipovitellins from Schizocosa malitiosa spider. Lipoproteins were isolated by ultracentrifugation and their lipids, proteins and fatty acids were analyzed by a combination of chromatography techniques and electrophoresis. Two lipovitellins of high and very high density, 42 and 11.1 % total lipids, respectively were isolated, the HDL named LV1 with density of 1.13 g/ml, and the VHDL named LV2, density of 1.24 g/ml. Sphingomyelin was the major components in both of them, representing 48.3 and 76% of total lipids, respectively. Major fatty acids were 18:2>18:1>16:0>18:0 in LV1, and 16:0>18:1>18:2>18:0 in LV2. Native LV1 has a MW of 559 kDa, and 3 apolipoproteins of 116, 87 and 42 kDa. Native LV2 is more heterogeneous with 5 particles of MW 492, 405, 293, 213 and 48 kDa. The 5 have the same apolipoprotein composition with 4 subunits of 135, 126, 109 and 70 kDa, though in different ratios. The description and characterization of LV1 and LV2, the first egg lipoproteins described in the family Lycosidae, lead to get an insight into molecular and biochemical aspects of such a large group as spiders.