Magnetic field-assisted gene delivery into skeletal muscle derived cells. Use of the Green Fluorescent Protein as a reporter

PEREYRA A; GOYA RG; MOREL GR; MYKHAYLYK O; DELBONO O; TAYLOR J; HEREÑU CB

Buenos Aires

Workshop; The Third South American Workshop and International Gregorio Weber Conference on New Trends in Advanced Fluorescence Microscopy Techniques; 2011

Fluorescence Fundation

The goal of the magnetic field-assisted delivery is to concentrate, in target areas, magnetic nanoparticles (MNPs) functionalized with the molecule of interest, by the use of an external magnetic fields generator-applicator. The stable and nontoxic Fe3O4 MNPs ? Recombinant Adenoviral vectors (RAd) complexes have become an important and interesting tool for gene transfer in the area of Biomedical Sciences and they have allowed us to develop magnetic gene targeting in C2C12 cell line. Here, the use of the fluorescent reporter protein -GFP- incorporated to the RAd´s genome provides a pivotal tool for the screening of gene transfer efficiency