Islet changes induced by increased fructose intake: increased B-cell apoptosis and islet fat deposit

REBOLLEDO O; MARRA CA; DEL ZOTTO H; BORELLI MI; MAIZTEGUI B; GAGLIARDINO JJ

Natal, Rio Grande do Norte

Jornada; ISG 2011. Annual Meeting of the EASD Islet Study; 2011

EASD (European Association for the Study of Diabetes) Islet Study Group

Background/aims: While the pro apoptotic effect of extracellular fatty acids concentration -particularly the saturated ones- on β-cells has been largely demonstrated, there are no clear evidences on the possible effect of their abnormal deposit in the islets. Thus, we have measured the amount and fatty acid (FA) composition of islet triglyceride (TG) content of pancreatic islets from normal rats fed with a fructose rich diet (F), experimental model known to affect the size, composition and function of fat stores as well as the β-cell mass and function. Methods: Normal adult Wistar rats were fed for 3 weeks with a commercial diet with (F) or without 10% fructose in the drinking water (C). Glucose (G), TG, free fatty acids (FFA) and insulin (I) plasma levels and the HOMA-IR index were measured. G was also measured after a glucose load. TG content and FA composition were measured in abdominal fat (AF), pancreatic islets, liver and plasma lipoproteins and its sub fractions (PLP); pancreas irnmunomorphometric analysis was also performed. Results: F rats had significantly (p