INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of mandarin essential oil (MO). Effect on cholesterol metabolism in Hep G2
Autor/es:
MANASSERO CARLOS ALBERTO, GIROTTI J., MIJAILOVSKY S., GARCIA DE BRAVO M., POLO M
Lugar:
Potrero de los Funes
Reunión:
Congreso; XLVII Reunión Anual de SAIB; 2011
Resumen:
CHARACTERIZATION OF MANDARIN ESSENTIAL OIL(MO). EFFECT ON CHOLESTEROL METABOLISM IN HepG2Manassero CA, Girotti J, Mijailovsky S, García de Bravo M, Polo M.INIBIOLP (UNLP-CONICET CCT La Plata) Fac. de Cs. Médicas. UNLP. La Plata. E-mail: charly.fce@hotmail.comEssential oils are fragrant volatile oils found in specialized plant cells or structures. Among their components, monoterpenes are phytochemicals that could have antitumor activity because of their multiple effects on mevalonate pathway. We have shown that MO (Citrus reticulata Blanco) and limonene (LM) exertantiproliferative activity against HepG2 cells with an IC50 = 0.063 µl/ml and 0.15 µl/ml respectively. The aim of this work was to determine the volatile organic compounds (VOCs) of MO and to study the effect of MO and LM on cholesterol homeostasis in HepG2 cells. VOCs of MO were determined by capillary gaschromatography coupled to mass spectrometry. Cholesterogenesis, cholesterol content, export and import were determined using radioactive precursors and biochemical methods. More than 89% of MO volatile fraction corresponds to limonene. Linalool, n-decanal, perill aldehyde, citronellol and dodecanal are minor components. Endogenous synthesis and incorporation of exogenous cholesterol are decreased in both treatments with a cholesterol content maintained or slightly increased. Cholesterol export diminished only in LM treated cells. Our data suggest that the antiproliferative activity of MO and LM in HepG2 cells would not be related to depletion of endogenous cholesterol pool and the effect on cholesterogenesis would be the consequence of the inhibition of cell growth.