INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of mandarin essential oil (MO). Effect on cholesterol metabolism in Hep G2
Autor/es:
MANASSERO CARLOS ALBERTO, GIROTTI J., MIJAILOVSKY S., GARCIA DE BRAVO M., POLO M
Lugar:
Potrero de los Funes
Reunión:
Congreso; XLVII Reunión Anual de SAIB; 2011
Resumen:
CHARACTERIZATION OF MANDARIN ESSENTIAL OIL(MO).  EFFECT ON  CHOLESTEROL METABOLISM  IN HepG2Manassero CA, Girotti J, Mijailovsky S, García de Bravo M, Polo M.INIBIOLP (UNLP-CONICET CCT La Plata) Fac. de Cs. Médicas. UNLP. La Plata. E-mail: charly.fce@hotmail.comEssential oils are fragrant volatile oils found in specialized plant cells  or  structures.  Among  their  components,  monoterpenes  are phytochemicals that could have antitumor activity because of their multiple effects on mevalonate pathway. We have shown that MO (Citrus reticulata Blanco) and limonene (LM) exertantiproliferative activity against HepG2 cells with an IC50 = 0.063 µl/ml  and  0.15  µl/ml respectively. The  aim  of  this  work  was  to determine the volatile organic compounds (VOCs) of MO and to study the effect of MO and LM on cholesterol homeostasis in HepG2 cells. VOCs of MO were determined by capillary gaschromatography coupled to mass spectrometry. Cholesterogenesis, cholesterol  content,  export  and  import  were  determined  using radioactive precursors and biochemical methods. More than 89% of MO volatile fraction corresponds to limonene. Linalool, n-decanal, perill aldehyde, citronellol and dodecanal are minor components. Endogenous synthesis and incorporation of exogenous cholesterol are  decreased  in  both  treatments  with  a  cholesterol  content maintained  or  slightly  increased.  Cholesterol  export  diminished only in LM treated cells. Our data suggest that the antiproliferative activity  of MO  and LM in HepG2  cells would  not  be related to depletion  of  endogenous  cholesterol  pool  and  the  effect  on cholesterogenesis would be the consequence of the inhibition of cell growth.