Synthesis and cell internalization of magnetite nanoparticles for magnetic hyperthermia applications

DE SOUSA; GOYA RODOLFO

Congreso; HK 2011-Humboldt Kolleg; International Conference on Physics; 2011

AFA

Magnetic hyperthermia treatment promises to be a viable alternative or complementary procedure for localized cancerous tumors therapy. The treatment consists of locally injecting magnetic nanoparticles (MNP) into the tumor and exposing it to an oscillating magnetic field in the radio frequency range. When exposed to this field, the MNP dissipates energy causing a temperature increase in the zone that contains nanoparticles, inducing tumor cell death by apoptosis at or above 42 °C. MNP energy dissipation is characterized by the specific power loss parameter (SLP) which expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the energy dissipation is characterized by the specific power loss parameter (SLP) which expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the MNP energy dissipation is characterized by the specific power loss parameter (SLP) which expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the loss parameter (SLP) which expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the -1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the from human lung adenocarcinoma for two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows thels follows the endocytic pathway. It is observed that MNP concentration inside cells is larger and more densely packed for the larger MNP concentration. Confocal, atomic force and transmission electron microscopies were used to image the in vitro uptake of nanoparticles by A549 cells. more densely packed for the larger MNP concentration. Confocal, atomic force and transmission electron microscopies were used to image the in vitro uptake of nanoparticles by A549 cells. s is larger and more densely packed for the larger MNP concentration. Confocal, atomic force and transmission electron microscopies were used to image the in vitro uptake of nanoparticles by A549 cells.