Linalool affects mevalonate pathway and cholesterol homeostasis in HepG2 and A549 cells

RODENAK KLADNIEW B; POLO M; GARCÍA DE BRAVO M

Potrero de los Funes, San Luis.

Congreso; XLVII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Linalool affects mevalonate pathway and cholesterol homeostasis in HepG2 and A549 cells Rodenak Kladniew Boris, Polo Mónica and García de Bravo Margarita INIBIOLP (UNLP-CONICET CCT La Plata) Fac. de Cs. Médicas. UNLP. La Plata Monoterpenes including geraniol, limonene, linalool (LN), menthol, are non-nutritive components of diet present in essential oils of citrus, rosemary, basil, mint and other herbs. These 10-carbon isoprenoids are derived from the mevalonate pathway (MP) in plants but are not produced by mammals and fungi. In previous studies we demonstrated that LN inhibits cell proliferation (CP) in HepG2 (IC50 = 1550 uM) and A549 (IC 50 = 1100 uM) cells, phenomenon attributed to the multiple pharmacological effects of monoterpenes on the MP. The aim of this work was to study the effect of LN on the MP and cholesterol (Cho) homeostasis. Cells were treated with increasing concentrations of LN that do not inhibit significantly CP in HepG2 (0-1000 uM) and A549 (0-700 uM) cells. Incorporation of 14C-acetate into MP intermediates separated by TLC was determined. Acording to the results we chose an LN concentration (200 uM) that inhibits cholesterol synthesis (ChS) in both cell lines to assess Cho import (ChI), Cho export (ChE) and its cellular content (ChC). Our data show that ChS decreases (30 and 24%), ChI increases (19% and 49%) and ChC decreases (18%) in HepG2 and A549 cells, respectively. We conclude that in conditions where the cell proliferation was not affected, linalool significantly altered the choleterogenesis and its cholesterol homeostasis.