INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Application of proteomic analysis in the search for biomarkers of oil pollution in the freshwater prawn Macrobrachium borellii
Autor/es:
PASQUEVICH M.Y.; DREON M. S.; H. HERAS
Lugar:
Buenos Aires
Reunión:
Workshop; Fronteras de Biociencias; 2012
Resumen:
Oil spills are common around the world and a major source of estuarine oil pollution. However, little is known of how it affects species in the community. In order to study the biological effect of a freshwater oil spill on organisms and identify potential pollution biomarkers, we analyzed male and female subproteomes of the prawn Macrobrachium borellii exposed to the water-soluble fraction (WSF) of crude oil in a laboratory bioassay. To this aim, protein mixtures were extracted from midgut gland prawns exposed for 7 days to a sublethal concentration of WSF (0.6 ppm), mostly a mixture of low-boiling aromatic hydrocarbons. Midgut gland proteins from non exposed prawns were used as control. Protein extracts were subsequently subject to 2D gel electrophoresis and spots from exposed and control animals were compared. Differentially-expressed proteins were analyzed by ESI-TOF/TOF mass spectrometry and their identity assigned in a MASCOT search mined against all known proteins. The presence of several proteins over- and underexpressed was observed in both sexes. The analysis of differentially expressed proteins in prawns exposed to hydrocarbons provided the identification of proteins which could be employed as potential biomarkers, highlighting the usefulness of 2DE-based proteomic analysis in pollution studies.