INIBIOLP 05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of water-soluble crude oil hydrocarbon exposure on protein expression of a freshwater crustacean
Autor/es:
PASQUEVICH, M.Y.; DREON, M.S.; HERAS, H.
Lugar:
Potrero de los Funes
Reunión:
Congreso; XLVII Reunión SAIByBM; 2011
Institución organizadora:
SAIByBM
Resumen:
Oil spills are common worldwide and a major source of estuarine oil pollution. However, little is known on how it affects species in the community. To study the effect of a freshwater oil spill on organisms and identify potential pollution biomarkers, we analyzed male and female subproteomes of the prawn Macrobrachium borellii exposed to the water-soluble fraction (WSF) of crude oil in a laboratory bioassay.
Prawns were exposed to a sublethal concentration of WSF (0.3 ppm) for 7 days and proteins extracted from midgut gland. Extracts were subsequently subjected to 2D gel electrophoresis and spots from exposed and control gels were compared. Differentially-expressed proteins were analyzed by ESI-TOF/TOF mass spectrometry and their identity assigned in a MASCOT search mined against all known proteins. The presence of several proteins over- and underexpressed was observed in both sexes. These differentially expressed proteins could be employed as potential biomarkers, highlighting the usefulness of 2DE-based proteomic analysis in pollution studies.
