Lipid dynamics of arachnid lipoproteins

LAINO ALDANA; GARCIA FERNANDO; HERAS HORACIO; CUNNINGHAM MÓNICA

Puerto Madryn, Chuut

Congreso; XLVI Reunión anual-sociedad argentina de Investigación en Bioquímica y Biología Molecular; 2010

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

It has been already reported that hemolymph lipíds in the spíder P. pythagoricus are transported by HDLl and VHDL lipoproteins. In the present work we studied in vitro´ the lipid transfer among intestinal diverticula (ID), princípal organ involved in lipíd biosynthesis, and either hemolymph (HL) or purified lipoproteíns as well as between HL lipoproteíns. ID and HL were labeled in vivo with 14C-palmitic acid. In vitro incubations were between ID and HL, as well as between ísolated lipoproteins and ID were performed, being lipid transfer quantified by radio-TLC. . Lípoproteíns (VHDLand ín a mínor extent by HDL1) released 76% ofíts lipids to ID. Isolated lipoproteins showed the same behavior to that observed wíth whole hemolymph. Lipid uptake in hemolymph was greater in HDLI (67%), though it was similar when the isolated lipoproteíns (50% each) were assayed. Lipid uptake and release in these systems were found to be constant within a wide range ofstarting lipíd labe!´ Intestinal diverticula were found to retain free fatty acíds and tryacilglicerides as only 28% of the label were transferred to lipoproteins while 76% was taken up from HL. A similar trend was observed regarding lipoprotein PL