INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Essential fatty acid deficiency in erythrocyte and nasal mucosa in children with cystic fibrosis
Autor/es:
D'ALESSANDRO VíGINIA; FERNANDEZ ADRIANA; MARIN MARIA CRISTINA; MARTINEZ MARIA INES; VERCESI SABRINA.; OTERO MARíA DEL CARMEN; TACCONI DE ALANIZ MARíA J; SEGAL EDGARDO
Lugar:
Baltimore
Reunión:
Congreso; 24th Annual North American Cystic Fibrosis Conference (NACFC); 2010
Institución organizadora:
Cystic Fibrosis Foundation
Resumen:
ABSTRACT BODY: Introduction: Essential fatty acid deficiency (EFAD) has been well documented in cystic fibrosis (CF) and pancreatic insufficiency, and may be involved in inflammatory response. Several studies indicated that these deficiencies are independent of nutritional status, but they may be related to CF regulated tissues.Objective: To determine the essential fatty acid profile in nasal epithelial and erythrocyte membrane in patients with CF controlled in our Center.Patients and Methods: Cross sectional study. We enrolled patients with CF (n:11) > 6 years old, with genotype and mild to moderate lung disease and pancreatic insufficiency, and a healthy control group (n:10). The mean age was 10.1 years for both groups and Z score BMI for CF group -0.57. None of the CF patients had liver disease, signs of infection or nutritional supplements. Blood samples and nasal epithelial scrapings were drawn. Total lipids of plasma and nasal mucosa were extracted by chloroform-methanol 2:1 v/v (Folch method). Fatty acid methyl esters were analyzed by gas-liquid chromatography in a capillary column, and the fatty acid pattern was determined. Statistical Method: ANOVA test.Results: Children with CF showed lower level of essential fatty acids compared to control, especially in Ón-6 and in total polyunsaturated fatty acids in nasal mucosa and erythrocyte membrane.Conclusion: EFAD seems apparent in CF children when compared to a healthy control sample. The importance of these findings related to pulmonary disease should be determined, as well as the role of mutant CFTR in essential fatty acid deficiency.   Erythrocytes Nasal mucosa   CF Control CF Control Ópolyunsaturated 30.8 ± 2.2 38.8 ± 1.2 * 11.4 ± 0.8 15.7 ± 1.3 * Ón-6 26.5 ± 2.3 33.7 ± 1.2 * 7.9 ± 0.9 11.4 ± 1.5 * Ón-3 4.2 ± 0.6 4.6 ± 0.2 2.8 ± 0.4 3.3 ± 0.5 Ratio 20:4n-6/22:6n-3 6.8 ± 0.5 5.5 ± 0.2 * 1.9 ± 0.2 2.7 ± 0.8 Values are mean ± SEM. * p < 0.05 relative to control