INIBIOLP 05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect o human milk pasteurization on total fat content.
Autor/es:
MARIN MARIA CRISTINA; SANJURJO ADRIANA; SAGER GUSTAVO; ALANIZ MARIA J. TACCONI DE
Lugar:
Itapecerica da Serra
Reunión:
Congreso; Latinoamerica Society for Pediatric Research XLVIII Annual Meeting; 2010
Institución organizadora:
Latinoamerica Society for Pediatric Research
Resumen:
Background: Human milk is pasteurized to reduce contamination, particularly in banked milk used to feed preterm infants. Objective: This comparative study was designed to determine the effect of pasteurization on human milk fat content, and on fatty acid composition. Human milk is pasteurized to reduce contamination, particularly in banked milk used to feed preterm infants. Objective: This comparative study was designed to determine the effect of pasteurization on human milk fat content, and on fatty acid composition.
Methods: Fifty-six milk samples from 28 mothers donors at the Milk Bank were collected and analyzed before pasteurization, and after pasteurization (Holder method, 62.5oC, 30 min). Total lipids were extracted by Folch method, using chloroform: methanol (2:1v/v), and gravimetrically quantified. Fatty acids were determined by gas-liquid chromatography (GLC). Results: There were not significant differences between before and after pasteurization regarding fatty acid content (linoleic acid 15.70.6 vs.15.80.5), calculated indexes (total polyunsaturated 19.10.5 vs. 19.00.6) and total lipid content (3.10.6 g/dl and fatty acid composition was not affected by pasteurization.
