Membrane Organization and Regulation of Cellular Cholesterol

MARIA S. JAUREGUIBERRY; M. ALEJANDRA TRICERRI; SUSANA A. SANCHEZ; HORACIO A. GARDA; GABRIELA S. FINARELLI; MARINA C. GONZALEZ; OMAR J. RIMOLDI

JOURNAL OF MEMBRANE BIOLOGY

SPRINGER

Lugar: New York; Año: 2010 vol. 234 p. 183 - 194

0022-2631

An excess of intracellular free colesterol (Chol) is cytotoxic, and its homeostasis is crucial for cell viability. Apolipoprotein A-I (apoA-I) is a highly efficient Chol acceptor because it activates complex cellular pathways that tend to mobilize and export Chol from celular depots. We hypothesize that membrane composition and/or organization is strongly involved in Chol homeostasis. To test this hypothesis, we constructed a cell line overexpressing stearoyl coenzyme A (CoA) desaturase (SCD cells), which modifies plasma membrane (PM) composition by the enrichment of monounsaturated fatty acids, and determined this effect on membrane properties, cell viability, and Chol homeostasis. PM in SCD cells has a higher ratio of phospholipids to sphingomyelin and is slightly enriched in Chol. These cells showed an increase in the ratio of cholesteryl esters to free Chol; they were more resistant to Chol toxicity, and they exported more caveolin than control cells. The data suggest that cell functionality is preserved by regulating membrane fluidity and Chol exportation and storage.