INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Glycoxidative stress-induced damage on lipid profile in a fructose-enriched diet model of insulin resistence in rats.
Autor/es:
GARCÍA M.E.; MARRA, CARLOS ALBERTO; REBOLLEDO, OSCAR
Revista:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Duesseldorf; Año: 2009 vol. 33 p. 1 - 8
ISSN:
0003-9861
Resumen:
The metabolic syndrome, an association of an insulin-resistant state (IR), alterations in glucose tolerance or type 2 diabetes, central obesity, hypertension and dyslipidemia, is quickly developing in human populations consuming high caloric diets together with  a tendency to sedentary behaviors (1, 2). These abnormalities constitute a high risk for developing a cardiovascular disease, the main cause of morbidity and mortality in developed and developing countries. Feeding rats with a high sucrose (SRD) or fructose diet (FRD) induce early changes in glucose and lipid metabolism resembling the profile of the human metabolic syndrome thus providing a useful model to study its consequences as well as to design different preventive strategies (3 – 7). This relatively short demanded for experimental animals submitted to unbalanced diets to become insulin-resistant is of particular importance to study the developing of insulin resistance because many patients are not properly diagnosed or do not receive appropriate treatment for their IR during long periods of time before developing a type 2 diabetes ( 8 ). It is also known that a FRD promotes oxidative damage both reducing antioxidant defenses and enhancing production of reactive oxygen species (ROS) (9 – 15). It has been documented that the states of IR are associated with an increased production of ROS generation an oxidative stress that contributes to the development of chronic complications of diabetes.(16 – 18) Alterations in liver, heart, kidney, abdominal adipose tissue lipid metabolism and in the plasma lipid profile have been reported in rats fed a FRD. The IR state enhances esterification of free fatty acids (FFAs) in the liver resulting in an elevation in plasma very low-density lipoproteins (VLDL)  and the AAT increases the release of FFAs (15,19). Conversely the lipid composition of plasma lipoproteins and the state of peroxidation of their FFAs have not been fully assessed. We have currently studied the effects of a short-term administration (3weeks) of a FRD to normal rats during the developing of an early insulin-resistant state on the plasma lipid profile, the composition and quality of peroxidation of FFAs attained in the different lipoproteins as well as their kinetics of oxidation. Besides, atherogenic risk scores in the FRD population with respect to the control group were analyzed.