INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
Autor/es:
LACUNZA, EZEQUIEL; MEMOLI, DOMENICO; QUIROGA IY; GONZALEZ-BARO, MARIA R.; MONTANARO, MA; RIZZO, F; SALVATI, ANNAMARIA; GUILLOU, H; HENNING, MARÍA FLORENCIA; WEISZ, A; ABBA, MC; PELLON MAISON, M
Revista:
Oncotarget
Editorial:
Albany, N.Y. : Impact Journals
Referencias:
Año: 2018 vol. 9 p. 28141 - 28154
ISSN:
1949-2553
Resumen:
Glycerol-3-phosphate acyltransferase-2 is a member of ?cancer-testis gene? family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ?differentiation tRNAs?, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.