INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Riboflavin induces Metarhizium spp. to produce conidia with elevated tolerance to UV-B, and upregulates photolyases, laccases and polyketide synthases genes
Autor/es:
PAIXÃO, FLÁVIA R. S.; PEDRINI, NICOLÁS; PEREIRA-JUNIOR, RONALDO A.; HUARTE-BONNET, CARLA; ROBERTS, DONALD W.; LUZ, CHRISTIAN; FERNANDES, ÉVERTON K. K.
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2018 vol. 125 p. 159 - 171
ISSN:
1364-5072
Resumen:
Aims: The effect of nutritional supplementation of two Metarhizium specieswith riboflavin (Rb) during production of conidia was evaluated on(i) conidial tolerance (based on germination) to UV-B radiation and on(ii) conidial expression following UV-B irradiation, of enzymes known to beactive in photoreactivation, viz., photolyase (Phr), laccase (Lac) and polyketidesynthase (Pks).Methods and Results: Metarhizium acridum (ARSEF 324) and Metarhiziumrobertsii (ARSEF 2575) were grown either on (i) potato dextrose agar medium(PDA), (ii) PDA supplemented with 1% yeast extract (PDAY), (iii) PDAsupplemented with Rb (PDA+Rb), or (iv) PDAY supplemented with Rb(PDAY+Rb). Resulting conidia were exposed to 8667 mW m2 of UV-BQuaite-weighted irradiance to total doses of 39 or 624 kJ m2. Some conidiaalso were exposed to 16 klux of white light (WL) after being irradiated, or not,with UV-B to investigate the role of possible photoreactivation. Relativegermination of conidia produced on PDA+Rb (regardless Rb concentration) oron PDAY and exposed to UV-B was higher compared to conidia cultivated onPDA without Rb supplement, or to conidia suspended in Rb solutionimmediately prior to UV-B exposure. The expression of MaLac3 and MaPks2for M. acridum, as well as MrPhr2, MrLac1, MrLac2 and MrLac3 forM. robertsii was higher when the isolates were cultivated on PDA+Rb andexposed to UV-B followed by exposure to WL, or exposed to WL only.Conclusions: Rb in culture medium increases the UV-B tolerance ofM. robertsii and M. acridum conidia, and which may be related to increasedexpression of Phr, Lac and Pks genes in these conidia.Significance and Impact of the Study: The enhanced UV-B tolerance ofMetarhizium spp. conidia produced on Rb-enriched media may improve theeffectiveness of these fungi in biological control programs.