Human apolipoprotein A-I Gly26Arg stimulation of inflammatory responses via NF-kB activation: Potential roles in amyloidosis?

RIOS J.; GUILLERMO R. SCHINELLA; RIOS J.; GUILLERMO R. SCHINELLA; ANDUJAR, I; M. ALEJANDRA TRICERRI; NAHUEL A. RAMELLA; ANDUJAR, I; ROSU SILVANA A.; M. ALEJANDRA TRICERRI; NAHUEL A. RAMELLA; ROSU SILVANA A.

PATHOPHYSIOLOGY (AMSTERDAM)

Elsevier B.V.

Año: 2018 vol. 18 p. 397 - 404

0928-4680

The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not completely understood, neither the pathways that determine clinical manifestations associated to systemic protein deposition in organs such as liver, kidney or heart. About twenty natural variants of apoA-I were described as inducing amyloidosis, but the mechanisms driving their aggregation and deposition are still unclear. We previously identified that the mutant Gly26Arg but not Lys107-0 induced release of cytokines and reactive oxygen species from cultured RAW 264.7 murine macrophages, suggesting that part of the pathogenic pathway could be the eliciting of an inflammatory signal. In this work we reached deep insight into this mechanism and determined that Gly26Arg induced a specific proinflammatory cascade involving activation of NF-B and its translocation into the nucleus. These findings suggest that some, but not all apoA-I natural variants might promote a pro-oxidant microenvironment which could in turn result in oxidative processing of the variants into a misfolded conformation.