INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Collision-mediated transfer of fatty acids from Yarrowia lipolytica sterol carrier protein 2 to phospholipid membranes.
Autor/es:
FALOMIR LOCKHART, L.J; BURGARDT, N.I; FERREYRA, R.G; CEOLIN, M; ERMÁCORA, M.R; CÓRSICO, B.
Revista:
BIOPHYSICAL JOURNAL
Editorial:
CELL PRESS
Referencias:
Año: 2009 vol. 97 p. 248 - 256
ISSN:
0006-3495
Resumen:
ABSTRACT Sterol carrier protein 2 (SCP2) is an intracellular protein domain found in all forms of life. It was originally identified
as a sterol transfer protein, but was recently shown to also bind phospholipids, fatty acids, and fatty-acyl-CoA with high affinity.
Based on studies carried out in higher eukaryotes, it is believed that SCP2 targets its ligands to compartmentalized intracellular
pools and participates in lipid traffic, signaling, and metabolism. However, the biological functions of SCP2 are incompletely characterized
and may be different in microorganisms. Herein, we demonstrate the preferential localization of SCP2 of Yarrowia
lipolytica (YLSCP2) in peroxisome-enriched fractions and examine the rate and mechanism of transfer of anthroyloxy fatty
acid from YLSCP2 to a variety of phospholipid membranes using a fluorescence resonance energy transfer assay. The results
show that fatty acids are transferred by a collision-mediated mechanism, and that negative charges on the membrane surface are
important for establishing a collisional complex. Phospholipids, which are major constituents of peroxisome and mitochondria,
induce special effects on the rates of transfer. In conclusion, YLSCP2 may function as a fatty acid transporter with some degree
of specificity, and probably diverts fatty acids to the peroxisomal metabolism.Yarrowia
lipolytica (YLSCP2) in peroxisome-enriched fractions and examine the rate and mechanism of transfer of anthroyloxy fatty
acid from YLSCP2 to a variety of phospholipid membranes using a fluorescence resonance energy transfer assay. The results
show that fatty acids are transferred by a collision-mediated mechanism, and that negative charges on the membrane surface are
important for establishing a collisional complex. Phospholipids, which are major constituents of peroxisome and mitochondria,
induce special effects on the rates of transfer. In conclusion, YLSCP2 may function as a fatty acid transporter with some degree
of specificity, and probably diverts fatty acids to the peroxisomal metabolism.(YLSCP2) in peroxisome-enriched fractions and examine the rate and mechanism of transfer of anthroyloxy fatty
acid from YLSCP2 to a variety of phospholipid membranes using a fluorescence resonance energy transfer assay. The results
show that fatty acids are transferred by a collision-mediated mechanism, and that negative charges on the membrane surface are
important for establishing a collisional complex. Phospholipids, which are major constituents of peroxisome and mitochondria,
induce special effects on the rates of transfer. In conclusion, YLSCP2 may function as a fatty acid transporter with some degree
of specificity, and probably diverts fatty acids to the peroxisomal metabolism.