INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Oxidative stress biomarkers and hormonal profile in human patients undergoing varicocelectomy
Autor/es:
HURTADO DE CATALFO, GRACIELA; RANIERI-CASILLA, ADALBERTO; MARRA, FERNANDO; ALANIZ, MARÍA J. TACCONI. DE; MARRA, CARLOS ALBERTO
Revista:
International Journal of Andrology
Editorial:
Blackwell Publishing
Referencias:
Lugar: Copenhagen, Dennmark; Año: 2007 vol. 30 p. 519 - 530
Resumen:
Summary
The aetiology of varicocele is multifactorial although hormonal imbalance and
oxidative stress play a key role in the progression of illness. No conclusive evidence
has been presented previously, describing the changes in these two factors
and the evolution of patients after varicocelectomy. Semen characteristics
and hormonal profile were analysed in 36 infertile men with unilateral left
varicocele and 33 age-paired controls (proved to be fertile men), after careful
inclusion/exclusion selection criteria. Liposoluble and hydrosoluble antioxidants,
oligoelements and enzyme activities of the antioxidant defence system
were also determined in plasma and erythrocyte from antecubital and spermatic
veins, and in spermatozoa. Data were compared between groups at different
times before and after varicocelectomy. Decreased levels of liposoluble
and hydrosoluble antioxidants and increased activities of the antioxidant
defence system enzymes were observed in patients compared with controls.
Varicocelectomy normalized this condition at different post-surgical times. Levels
of Zn and Se in seminal plasma, protein carbonyls and fragmented DNA
remained elevated up to 1 month after surgery. Luteinizing and follicle stimulating
hormone concentrations exhibited a biphasic behaviour while testosterone
was diminished in patients but normalized soon after varicocelectomy. The
results clearly demonstrate the link between the antioxidant defence system,
hormonal status and semen characteristics along the post-varicocelectomy period.
We suggest that oxidative biomarkers may be appropriate in controlling
the evolution of post-varicocelectomy patients, and antioxidant supplementation
may improve the clinical condition of infertile men with varicocele.