INIBIOLP 05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
The CavBeta1a subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
Autor/es:
JACKSON TAYLOR; ANDREA S. PEREYRA; TAN ZHANG; MARIA LAURA MESSI; ZHONG-MIN WANG; CLAUDIA HEREÑÚ; PEI-FEN KUAN; OSVALDO DELBONO
Revista:
JOURNAL OF CELL BIOLOGY
Editorial:
ROCKEFELLER UNIV PRESS
Referencias:
Lugar: New York; Año: 2014 vol. 205 p. 829 - 846
ISSN:
0021-9525
Resumen:
Voltage-gated calcium channel (Cav) Beta subunits are auxiliary subunits to Cavs. Recent reports show CavBeta subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of CavBeta in muscle progenitor cells (MPCs). We found that CavBeta1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of CavBeta1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that CavBeta1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. CavBeta1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that CavBeta1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.
