Toxicological and biochemical response of the entomopathogenic fungus Beauveria bassiana after exposure to deltamethrin

L. FORLANI; M. P. JUAREZ; S. LAVARIAS; N. PEDRINI

PEST MANAGEMENT SCIENCE

JOHN WILEY & SONS LTD

Lugar: Londres; Año: 2014 vol. 70 p. 751 - 756

1526-498X

BACKGROUND The chemical control of the Chagas disease vector Triatoma infestans isendangered by the emergence of pyrethroid resistance. An effective alternative control tool is the use of the entomopathogenic fungus Beauveria bassiana. The effect of deltamethrin onfungal growth, gene expression and enzyme activity in relation to detoxification, antioxidantresponse and oxidative stress levels was studied to evaluate fungal tolerance to deltamethrin.RESULTS The mean inhibitory concentration (IC50) was 50 ¦Ìg deltamethrin/cm2.Cytochrome P450 genes were differentially expressed; cyp52X1 and cyp617N1 transcriptswere > 2-fold induced, followed by cyp655C1 (1.8-fold). Minor effects were observed ongenes encoding for other P450s, epoxide hydrolase and glutathione S-transferase (GST).Superoxide dismutase (SOD) genes showed induction levels ¡Ü 2, catalase (CAT) andglutathione peroxidase genes were also induced ~ 2-3-fold and < 2-fold, respectively. Theactivities of enzymes participating in the antioxidant defense system and phase IIdetoxification were also evaluated; SOD, CAT and GST activity showed significantdifferences with deltamethrin concentration. Lipid peroxidation levels and free prolinecontent were also altered.CONCLUSIONS B. bassiana GHA can be used combined with deltamethrin withoutsignificant metabolic detrimental effects. This combination will help optimizing the benefitsand increasing the efficacy of vector control tools.