Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases

MARÍA S. JAUREGUIBERRY; M. ALEJANDRA TRICERRI; SUSANA A. SANCHEZ; GABRIELA S. FINARELLI; MAURO A. MONTANARO; EDUARDO PRIETO; OMAR J. RIMOLDI

ACTA BIOCHIMICA ET BIOPHYSICA SINICA

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2014 p. 1 - 10

1005-9628

Experimental evidence has suggested that plasma membrane(PM)-associated signaling and hence cell metabolismand viability depend on lipid composition and organization.The aim of the present work is to develop a cell modelto study the endogenous polyunsaturated fatty acids(PUFAs) effect on PM properties and analyze its influenceon cholesterol (Chol) homeostasis. We have previouslyshown that by using a cell line over-expressing stearoyl-CoA-desaturase, membrane composition and organizationcoordinate cellular pathways involved in Chol efflux andcell viability by different mechanisms. Now, we expandedour studies to a cell model over-expressing both D5 and D6desaturases, which resulted in a permanently higher PUFAcontent in PM. Furthermore, this cell line showed increasedPM fluidity, Chol storage, and mitochondrial activity. Inaddition, human apolipoprotein A-I-mediated Chol removalwas less efficient in these cells than in the correspondingcontrol. Taken together, our results suggested that the cellfunctionality is preserved by regulating PM organizationand Chol exportation and homeostasis.