INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Endonuclear lipids of liver cells
Autor/es:
SABINA M. MATÉ, RODOLFO R. BRENNER, ANA VES-LOSADA
Revista:
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
Editorial:
National Research Council Canadá
Referencias:
Lugar: Canadá; Año: 2006 vol. 84 p. 459 - 468
ISSN:
0008-4212
Resumen:
       Lipids are not only components of cell nucleus membranes, but they are also found in the  membrane depleted - nuclei, fulfilling special functions. Currently, we have investigated the lipid composition of membrane depleted rat liver nuclei obtained by incubation with low Triton X -100 concentrations of 0.04 and 0.08%, which rendered it unaltered or hardly altered. Under these conditions »26% of proteins and »22% of phospholipids were recovered. The main phospholipids were phosphatidylcholine> phosphatidylethanolamine >phosphatidylinositol ³ phosphatidylserine and sphingomyelin. The fatty acid components of total lipids and phosphatidylcholine  were mainly unsaturated. ³40% belonged to the n-6 series being arachidonic ³25% and linoleic »15%; »40% corresponded to saturated acids and <10% were monoenoic. Endonuclear phosphatidylcholine was built up by 16 molecular species the most abundant being 18:0 - 20:4 (»32%), 16:0 - 20:4 (»19%), 16:0 - 18:2 (»13%), and 18:0 - 18:2 (»11%). The fatty acid composition, and phosphatidylcholine molecular species distribution in the membrane  depleted nucleus of rat liver, obtained by TX-100 treatment, showed patterns similar to the whole nucleus, mitochondria, microsomes and homogenate of the  parent liver cells, suggesting that endonuclear lipid pools composition would be mainly determined by a liver organ profile.