INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Biomarkers of prolonged exposure to microcystin-LR in mice
Autor/es:
SEDAN DANIELA; GIANUZZI L.; ROSSO L.; MARRA CARLOS ALBERTO; ANDRINOLO DARÍO
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012
ISSN:
0041-0101
Resumen:
The effects of prolonged exposure to
microcystins (MCs) on health are not yet sufficiently understood and this type
of poisoning is often undiagnosed. Even though chronic exposure has been linked
with liver cancer and alterations have been described in liver damage marker
enzymes in exposed populations, there are not profile parameters that indicate prolonged
exposure to microcystins.
The aim of this work is to determine, based on an animal
model of prolonged exposure to successive i.p. doses of 25 µg MC-LR/kg body weight,
several plasma parameters which could be useful as exposure biomarkers.
Hemoglobin (Hb) and methemoglobin
(MetHb) levels were determined on blood
samples. We also studied plasma levels of hydroperoxides
(ROOHs), α-tocopherol,
glutathione and lipid profile as
well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination
of MC-LR levels in liver, kidney,
plasma, urine and feces of treated mice was
carried out. We found that alteration in MetHb, ROOHs, glutathione,
α-tocopherol levels, SOD activity and plasma lipid profile, correlates with
those expected if the alteration derived from hepatic damage. The alterated
plasma paramenters together with MC-LR determination could be used as
biomarkers, helpful tools in screening and epidemiological studies.