INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Pesticide-induced decrease in rat testicular steroidogenesis is differentially prevented by lipoate and tocopherol
Autor/es:
ASTIZ, MARIANA; HURTADO DE CATALFO, GRACIELA E.; GARCÍA MN; GALLETTI S.; ERRECALDE, A.L.; ALANIZ, MARÍA J.T. DE; MARRA CARLOS ALBERTO
Revista:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2012
ISSN:
0147-6513
Resumen:
We have previously demonstrated that
the sub-chronic administration of low doses of dimethoate, glyphosate and zineb
to rats (i.p. 1/250 LD50, 3 times a week for 5 weeks) provoked
severe oxidative stress (OS) in testicles. These effects were also reflected in
plasma. Lipoic acid (LA) and α-Tocopherol are considered as antioxidants due to
its ability to neutralize reactive oxygenated species (ROS) and reset endogenous
antioxidant levels. To investigate the possible protective effect on
reproductive function, LA or Toc (i.p. 25, 50 and 100 mg/Kg) were administered simultaneously
with the pesticide mixture (PM) for 5 weeks. Both drugs prevented OS and the damage
to proteins and lipids caused by PM in a dose-dependent manner. The PM-induced increase
levels of prostaglandins E2 and F2a that was completely restored by LA but
not by Toc. Similarly, only LA was able to restore the inhibition of
testosterone production, the decrease of 3b- and 17b-hydroxysteroid dehydrogenases activities,
and the elevation of gonatropins (FSH and LH) levels produced by PM. Furthermore,
LA was more efficient than Toc in normalizing the histological alterations
produced by PM administration. In our experimental model LA displayed a higher protective
role against pesticide-induced damage than that observed by Toc administration.
Our results suggest that LA administration is a promising therapeutic strategy for
coping with disorders suspected to be caused by OS generators- such as
pesticides- in male reproductive system.