Multidimensional microscopy: A suitable technique to follow in vivo the interactions between biodegradable biomaterials and cells.

ALVAREZ, FLORENCIA; LOZANO PUERTO, R; PEREZ MACEDA, B; GRILLO, C; FERNÁNDEZ LORENZO DE MELE, M

Microscopy: advances in scientific research and education

Formatex

Año: 2014; p. 523 - 529

Biodegradable metallic materials have important applications in medicine for orthopaedic, dental and cardiovascular therapies. These metals interact with the biological medium and are easily corroded due to the presence of chlorides. During degradation micro-debris are released. Macrophages, cells involved in immune response, interact with debris in different complex ways. Multidimensional microscopy refers to the generation of multiple in vivo images of a sample over time. This technique is very appropriate to follow in vivo the degradation process of a biodegradable biomaterial and the interactions of the macrophages with the released debris. In this chapter we will describe the use of this technique to study the interaction of Mg microparticles (Mg-MPs with J774 macrophages, a mouse cell line. Formation of H2 bubbles, changes in the morphology of macrophages due to interactions through their membranes and pseudopodia, duplication and death of cells were detected. Images of multidimensional microscopy show the great capability of this technique to follow biological and physicochemical processes at real time.