Structural conformation and self-assembly process of p31-43 gliadin peptide in aqueous solution. Implications for celiac disease

HERRERA, MARÍA GEORGINA; BARRERA, EXEQUIEL; CHIRDO, FERNANDO; PRIETO, EDUARDO; GÓMEZ CASTRO, MARÍA FLORENCIA; PANTANO, SERGIO; DODERO, VERONICA ISABEL; HERRERA, MARÍA GEORGINA; BARRERA, EXEQUIEL; CHIRDO, FERNANDO; PRIETO, EDUARDO; PANTANO, SERGIO; GÓMEZ CASTRO, MARÍA FLORENCIA; DODERO, VERONICA ISABEL

FEBS JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Año: 2019

1742-464X

Celiac disease (CeD) is a highly prevalent chronic immune-mediated enteropathy developed in genetically predisposed individuals after ingestion of a group of wheat proteins (called gliadins and glutenins). The 13mer α-gliadin peptide, p31-43, induces proinflammatory responses, observed by in vitro assays and animal models, that may contribute to innate immune mechanisms of CeD pathogenesis. Since a cellular receptor for p31-43 has not been identified, this raises the question of whether this peptide could mediate different biological effects. In this work, we aimed to characterize the p31-43 secondary structure by different biophysical and in silico techniques. By dynamic light scattering and using an oligomer/fibril-sensitive fluorescent probe, we showed the presence of oligomers of this peptide in solution. Furthermore, atomic force microscopy analysis showed p31-43 oligomers with different height distribution. Also, peptide concentration had a very strong influence on peptide self-organization process. Oligomers gradually increased their size at lower concentration. Whereas, at higher ones, oligomers increased their complexity, forming branched structures. By CD, we observed that p31-43 self-organized in a polyproline II conformation in equilibrium with β-sheets-like structures, whose pH remained stable in the range of 3?8. In addition, these findings were supported by molecular dynamics simulation. The formation of p31-43 nanostructures with increased β-sheet structure may help to explain the molecular etiopathogenesis in the induction of proinflammatory effects and subsequent damage at the intestinal mucosa in CeD.