Resveratrol enhancement on survival of staphylococcus aureus under levofloxacin and photodynamic treatments

SCHILARDI, PATRICIA; MIÑAN, ALEJANDRO; FERNÁNDEZ LORENZO DE MELE, MÓNICA; LORENTE, CAROLINA; GASPAR TOSATO, MAIRA; THOMAS, ANDRÉS H.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

ELSEVIER SCIENCE BV

Año: 2017

0924-8579

Reactive oxygen species (ROS) are an efficient tool to eradicate microorganisms, due to the capacity of these species to damage almost all types of biomolecules and to kill cells. The increment in the resistance mechanisms to antibiotics leads to the exploration of new strategies to eliminate microorganisms that involves production of ROS, such as superoxide anion (O2?-) and hydrogen peroxide (H2O2). ROS are produced during several antimicrobial treatments, including antibiotic and photodynamic therapies. Among natural antioxidants, resveratrol (RSV) is efficient to prevent damage from ROS, and every day more people incorporate it as a dietary or cosmetic supplement. However, the consequences of the administration of RSV during an antimicrobial treatment are unknown. To investigate possible antagonistic or synergistic effects of RSV during antibiotic therapy (levofloxacin, LVX) or a photodynamic therapy (visible radiation and methylene blue, MB), the elimination of S. aureus of a planktonic culture was evaluated in the presence of RSV. Results shows that the antimicrobial capacity of these therapies is significantly diminished when LVX or MB are co-administered with RSV, indicating that the consumption of RSV during an antimicrobial treatment must be, at least, warned. Moreover, considering that the ROS antimicrobial activity of antibacterial agents, the topical addition of RSV, may also affect the control of pathogens of the human body. The results presented in this article highlight the importance of the evaluation of possible antagonistic effect when an antimicrobial agent with ROS-mediated action is co-administrated with RSV.