A quantitative HPLC-MS method for the simultaneous determination of testosterone, 11-ketotestosterone and 11-â hydroxy-androstenedione in fish serum

MARTÍN BLASCO, PEDRO CARRIQUIRIBORDE, DAMIÁN MARINO, ALICIA E. RONCO, GUSTAVO M. SOMOZA

JOURNAL OF CHROMATOGRAPHY B

Elsevier

Año: 2009 vol. 877 p. 1509 - 1515

0378-4347

Abstract A simple and novel HPLC-MS method for the simultaneous quantification of 11-ketotestosterone, 11-â hydroxy-androstenedione and testosterone in fish serum was developed and validated. Separation was achieved on a C-18 column using a water–acetonitrile mobile-phase with a cycle time of 12 min. Ion detection was performed using ESI positive SIM at [M+H] (m/z 303, 303, 289). The linear ranges (0.2-50 ng/ml), limits of detection (0.1-0.2 ng/ml) and limits of quantification (0.2-0.5 ng/ml) were established. The method was validated by measuring the three androgens in goldfish sera, displaying comparable values to those reported by other analytical techniques (i.e RIA, EIA).