INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
Photodynamic therapy of HeLa cell cultures by using LED or laser sources
Autor/es:
PASQUALE, MIGUEL ANGEL; ETCHEVERRY, MARÍA EUGENIA; MARIO GARAVAGLIA
Revista:
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Editorial:
ELSEVIER SCIENCE SA
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 160 p. 271 - 277
ISSN:
1011-1344
Resumen:
The photodynamic therapy (PDT) on HeLa cell cultures was performed utilizing a 637 nm LED lampwith 1.06Wpower and m-tetrahydroxyphenyl chlorin (m-THPC) as photosensitizer and compared to a laser source emittingat 654 nmwith the same power. Intracellular placement of the photosensitizer and the effect of its concentration(CP), its absorption time(TA) and the illuminationtime(TI)were evaluated. It was observed that for CP N 40 μg/mland TA N 24 h, m-THPC had toxicity on cells in culture, even in the absence of illumination. For the other testedconcentrations, the cells remained viable if not subjected to illumination doses. No effect on cells was observedfor CP b 0.05 μg/ml, TA=48 h and TI=10 min and they continued proliferating. For drug concentrations higherthan 0.05 μg ml−1, further deterioration is observed with increasing TA and TI. We evaluated the viability of thecells, before and after the treatment, and by supravital dyes, and phase contrast and fluorescence microscopies,evidence of different types of cell death was obtained. Tetrazolium dye assays after PDT during different timesyielded similar results for the 637 nm LED lamp with an illuminance three times greater than that of the654 nm laser source. Results demonstrate the feasibility of using a LED lamp as alternative to laser source.Here the main characteristic is not the light coherence but achieving a certain light fluence of the appropriatewavelength on cell cultures.We conclude that the efficacy was achieved satisfactorily and is essential for convenience,accessibility and safety.