INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications
Autor/es:
M. SOLEDAD BELLUZOA, LARA F. MEDINAA, M. SUSANA CORTIZO.; AM CORTIZO
Revista:
ULTRASONICS SONOCHEMISTRY.
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 30 p. 1 - 8
ISSN:
1350-4177
Resumen:
In recent years, there has been an increasing interest in the design of biomaterials for cartilage tissueengineering. This type of materials must meet several requirements. In this study, we apply ultrasoundto prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose(CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the interpolymermacroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemicaldegradation of each component in order to optimize the experimental conditions for PEC compatibilization.Scaffolds obtained applying this methodology and scaffolds without ultrasound processingwere prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (byFTIR), stability and mechanical properties were analyzed. The swelling kinetics was studied and interpretedbased on the structural differences between the two kinds of scaffolds. In addition we evaluatethe possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstratethat the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improvedproperties compared with the simple mixture of the two polysaccharides. The test with murine macrophageRAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtainedunder ultrasound conditions could be useful in the cartilage tissue engineering field.